| |  Online-Ressource |
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| Verfasst von: | Gleißner, Christian A. [VerfasserIn]  |
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| | Shaked, Iftach [VerfasserIn] |
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| | Erbel, Christian [VerfasserIn] |
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| | Böckler, Dittmar [VerfasserIn] |
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| | Katus, Hugo [VerfasserIn] |
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| | Ley, Klaus [VerfasserIn] |
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| Titel: | CXCL4 downregulates the atheroprotective hemoglobin receptor CD163 in human macrophages |
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| Verf.angabe: | Christian A. Gleissner, Iftach Shaked, Christian Erbel, Dittmar Böckler, Hugo A. Katus, and Klaus Ley |
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| E-Jahr: | 2010 |
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| Jahr: | 2010 January 8 |
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| Umfang: | 19 S. |
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| Fussnoten: | Das PDF umfasst 19 Seiten ; Gesehen am 03.04.2023 |
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| Titel Quelle: | Enthalten in: Journal of banking and finance |
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| Ort Quelle: | Amsterdam : Elsevier North-Holland, 1977 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 106(2010), 1, Seite 203-211 |
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| ISSN Quelle: | 1872-6372 |
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| Abstract: | RATIONALE: CXCL4 is a platelet-derived chemokine that promotes macrophage differentiation from monocytes. Deletion of the PF4 gene that encodes CXCL4 reduces atherosclerotic lesions in ApoE(-/-) mice. - OBJECTIVE: We sought to study effects of CXCL4 on macrophage differentiation with possible relevance for atherogenesis. - METHODS AND RESULTS: Flow cytometry for expression of surface markers in macrophage colony-stimulating factor (M-CSF)- and CXCL4-induced macrophages demonstrated virtually complete absence of the hemoglobin scavenger receptor CD163 in CXCL4-induced macrophages. mRNA for CD163 was downregulated as early as 2 hours after CXCL4. CD163 protein reached a minimum after 3 days, which was not reversed by treatment of cells with M-CSF. The CXCL4 effect was entirely neutralized by heparin, which bound CXCL4 and prevented CXCL4 surface binding to monocytes. Pretreatment of cells with chlorate, which inhibits glycosaminoglycan synthesis, strongly inhibited CXCL4-dependent downregulation of CD163. Similar to recombinant CXCL4, releasate from human platelets also reduced CD163 expression. CXCL4-differentiated macrophages were unable to upregulate the atheroprotective enzyme heme oxygenase-1 at the RNA and protein level in response to hemoglobin-haptoglobin complexes. Immunofluorescence of human atherosclerotic plaques demonstrated presence of both CD68+CD163+ and CD68+CD163- macrophages. PF4 and CD163 gene expression within human atherosclerotic lesions were inversely correlated, supporting the in vivo relevance of CXCL4-induced downregulation of CD163. - CONCLUSIONS: CXCL4 may promote atherogenesis by suppressing CD163 in macrophages, which are then unable to upregulate the atheroprotective enzyme heme oxygenase-1 in response to hemoglobin. |
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| DOI: | doi:10.1161/CIRCRESAHA.109.199505 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
DOI: https://doi.org/10.1161/CIRCRESAHA.109.199505 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Animals |
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| | Antigens, CD |
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| | Antigens, Differentiation, Myelomonocytic |
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| | Apolipoproteins E |
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| | Atherosclerosis |
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| | Cell Differentiation |
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| | Gene Expression Regulation |
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| | Heme Oxygenase-1 |
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| | Hemoglobins |
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| | Humans |
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| | Macrophages |
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| | Mice |
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| | Mice, Knockout |
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| | Monocytes |
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| | Platelet Factor 4 |
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| | Receptors, Cell Surface |
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| | Receptors, Scavenger |
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| K10plus-PPN: | 1841074292 |
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| Verknüpfungen: | → Zeitschrift |
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CXCL4 downregulates the atheroprotective hemoglobin receptor CD163 in human macrophages / Gleißner, Christian A. [VerfasserIn]; 2010 January 8 (Online-Ressource)
