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| Verfasst von: | Bartkowiak, Kai [VerfasserIn]  |
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| | Mossahebi Mohammadi, Parinaz [VerfasserIn] |
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| | Gärtner, Sebastian [VerfasserIn] |
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| | Kwiatkowski, Marcel [VerfasserIn] |
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| | Andreas, Antje [VerfasserIn] |
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| | Geffken, Maria [VerfasserIn] |
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| | Peine, Sven [VerfasserIn] |
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| | Verpoort, Karl [VerfasserIn] |
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| | Scholz, Ursula [VerfasserIn] |
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| | Deutsch, Thomas M. [VerfasserIn] |
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| | Michel, Laura L. [VerfasserIn] |
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| | Schneeweiss, Andreas [VerfasserIn] |
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| | Thewes, Verena [VerfasserIn] |
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| | Trumpp, Andreas [VerfasserIn] |
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| | Müller, Volkmar [VerfasserIn] |
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| | Riethdorf, Sabine [VerfasserIn] |
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| | Schlüter, Hartmut [VerfasserIn] |
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| | Pantel, Klaus [VerfasserIn] |
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| Titel: | Detection and isolation of circulating tumor cells from breast cancer patients using CUB domain-containing protein 1 |
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| Verf.angabe: | Kai Bartkowiak, Parinaz Mossahebi Mohammadi, Sebastian Gärtner, Marcel Kwiatkowski, Antje Andreas, Maria Geffken, Sven Peine, Karl Verpoort, Ursula Scholz, Thomas M. Deutsch, Laura L. Michel, Andreas Schneeweiss, Verena Thewes, Andreas Trumpp, Volkmar Müller, Sabine Riethdorf, Hartmut Schlüter, and Klaus Pantel |
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| E-Jahr: | 2023 |
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| Jahr: | 16 March 2023 |
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| Umfang: | 18 S. |
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| Fussnoten: | Gesehen am 05.06.2023 |
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| Titel Quelle: | Enthalten in: Journal of proteome research |
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| Ort Quelle: | Washington, DC : ACS Publications, 2002 |
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| Jahr Quelle: | 2023 |
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| Band/Heft Quelle: | 22(2023), 4 vom: März, Seite 1213-1230 |
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| ISSN Quelle: | 1535-3907 |
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| Abstract: | In cancer metastasis, single circulating tumor cells (CTCs) in the blood and disseminated tumor cells (DTCs) in the bone marrow mediate cancer metastasis. Because suitable biomarker proteins are lacking, CTCs and DTCs with mesenchymal attributes are difficult to isolate from the bulk of normal blood cells. To establish a procedure allowing the isolation of such cells, we analyzed the cell line BC-M1 established from DTCs in the bone marrow of a breast cancer patient by stable isotope labeling by amino acids in cell culture (SILAC) and mass spectrometry. We found high levels of the transmembrane protein CUB domain-containing protein 1 (CDCP1) in breast cancer cell lines with mesenchymal attributes. Peripheral blood mononuclear cells were virtually negative for CDCP1. Confirmation in vivo by CellSearch revealed CDCP1-positive CTCs in 8 of 30 analyzed breast cancer patients. Only EpCam-positive CTCs were enriched by CellSearch. Using the extracellular domain of CDCP1, we established a magnetic-activated cell sorting (MACS) approach enabling also the enrichment of EpCam-negative CTCs. Thus, our approach is particularly suited for the isolation of mesenchymal CTCs with downregulated epithelial cancer that occur, for example, in triple-negative breast cancer patients who are prone to therapy failure. |
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| DOI: | doi:10.1021/acs.jproteome.2c00739 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1021/acs.jproteome.2c00739 |
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| | DOI: https://doi.org/10.1021/acs.jproteome.2c00739 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1847404626 |
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| Verknüpfungen: | → Zeitschrift |
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Detection and isolation of circulating tumor cells from breast cancer patients using CUB domain-containing protein 1 / Bartkowiak, Kai [VerfasserIn]; 16 March 2023 (Online-Ressource)
