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| Verfasst von: | Fuhler, Gwenny M. [VerfasserIn]  |
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| | Baanstra, Mirjam [VerfasserIn] |
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| | Chesik, Daniel [VerfasserIn] |
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| | Somasundaram, Rajesh [VerfasserIn] |
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| | Seckinger, Anja [VerfasserIn] |
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| | Hose, Dirk [VerfasserIn] |
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| | Peppelenbosch, Maikel P. [VerfasserIn] |
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| | Bos, Nico A. [VerfasserIn] |
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| Titel: | Bone marrow stromal cell interaction reduces Syndecan-1 expression and induces kinomic changes in myeloma cells |
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| Verf.angabe: | Gwenny M. Fuhler, Mirjam Baanstra, Daniel Chesik, Rajesh Somasundaram, Anja Seckinger, Dirk Hose, Maikel P. Peppelenbosch, Nico A. Bos |
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| E-Jahr: | 2010 |
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| Jahr: | 20 March 2010 |
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| Umfang: | 13 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 18.07.2023 |
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| Titel Quelle: | Enthalten in: Experimental cell research |
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| Ort Quelle: | Orlando, Fla. : Academic Press, 1950 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 316(2010), 11 vom: Juli, Seite 1816-1828 |
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| ISSN Quelle: | 1090-2422 |
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| Abstract: | CD138 (Syndecan 1) is a heparan sulfate proteoglycan that concentrates heparan sulfate-binding growth factors on the surface of normal and malignant plasma cells (multiple myeloma, MMC). Recent studies have shown the presence of a CD138-negative fraction of MMC within myelomatous bone marrow (BM). We employed kinome array technology to characterize this fraction at a molecular level, using a myeloma cell line model. Compared to CD138-positive cells, CD138-negative MMC showed (i) a reduced activity of kinases involved in cell cycle progression, in agreement with a decreased labeling index and (ii) reduced Rho signaling to F-actin. Interestingly, CD138 mRNA and protein expression was reduced upon interaction of MM cells with stromal cell lines and primary mesenchymal cultures, which was accompanied by the acquisition of an increased Bcl6/Blimp1 ratio. Co-culture induced an increased activity of kinases involved in adhesion and a decreased S-phase transition in both CD138-positive and -negative fractions. In addition, CD138-negative MMC demonstrated an increased STAT3 and ERK1/2 activation compared to CD138+ MMC, in agreement with a lower sensitivity to compound exposure. The presence of a less mature, more resistant CD138-negative myeloma cell fraction within bone marrow microniches might contribute to high incidence of relapse of Myeloma patients. |
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| DOI: | doi:10.1016/j.yexcr.2010.03.013 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.yexcr.2010.03.013 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S0014482710001205 |
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| | DOI: https://doi.org/10.1016/j.yexcr.2010.03.013 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Kinome |
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| | Multiple Myeloma |
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| | Signal transduction |
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| | Stroma |
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| | Syndecan-1 |
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| K10plus-PPN: | 1852904828 |
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| Verknüpfungen: | → Zeitschrift |
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Bone marrow stromal cell interaction reduces Syndecan-1 expression and induces kinomic changes in myeloma cells / Fuhler, Gwenny M. [VerfasserIn]; 20 March 2010 (Online-Ressource)
