Amplification of the epidermal-growth-factor-receptor gene correlates with different growth behaviour in human glioblastoma

• Verfasst von: Schlegel, Jürgen [VerfasserIn]
• Verfasst von: Merdes, Annette Ruth [VerfasserIn]
• Verfasst von: Stumm, Gabi [VerfasserIn]
• Verfasst von: Albert, Friedrich [VerfasserIn]
• Verfasst von: Forsting, Michael [VerfasserIn]
• Verfasst von: Hynes, Nancy [VerfasserIn]
• Verfasst von: Kiessling, Marika [VerfasserIn]
• Titel: Amplification of the epidermal-growth-factor-receptor gene correlates with different growth behaviour in human glioblastoma
• Verf.angabe: Jürgen Schlegel, Annette Merdes, Gabi Stumm, Fritz K. Albert, Michael Forsting, Nancy Hynes, Marika Kiessling
• E-Jahr: 1994
• Jahr: 2 January 1994
• Umfang: 6 S.
• Fussnoten: Gesehen am 12.09.2023
• Titel Quelle: Enthalten in: International journal of cancer
• Ort Quelle: Bognor Regis : Wiley-Liss, 1966
• Jahr Quelle: 1994
• Band/Heft Quelle: 56(1994), 1, Seite 72-77
• ISSN Quelle: 1097-0215
• DOI: doi:10.1002/ijc.2910560114
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1859355064

Abstract

The objective of our study was to determine the frequency of EGF-receptor-gene rearrangement in relation to tumour-growth behaviour in an unselected group of glioma patients. We investigated 73 glial tumours with different grades of malignancy (17 low-grade gliomas, 14 anaplastic variants, and 42 GBM) by Southern analysis, reverse transcriptase PCR (RT-PCR) amplification of mRNA, and Western analysis. An amplification of the EGF-receptor gene was present in 19/42 GBM but in only I anaplastic astrocytoma. By RT-PCR, 4/19 GBM with gene amplification showed a specific amino-terminal aberrant splice mutation of 801 bp in addition to undeleted mRNA. By Western analysis, 27/42 GBM showed expression of the EGF-receptor protein. Protein levels, however, varied among individual tumours. Four GBM containing an aberrant splice mutation exhibited an immunoreactive protein of 130 kDa MW in addition to the normal EGF-receptor protein p170. All GBM patients underwent surgery followed by a standard course of radiotherapy. Neuroradiological follow-up in 31/42 GBM patients consisted of bimonthly MRJ examinations. There was a statistically significant difference in the mean latency period until tumour regrowth of patients suffering from GBM with and without EGF-receptor-gene amplification (9 weeks vs. 32 weeks). Our data indicate more rapid tumour regrowth kinetics of GBM with amplified EGF receptor genes in vivo. © 1994 Wiley-Liss, Inc.