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| Verfasst von: | Wein, Frederik [VerfasserIn]  |
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| | Nkenmei-Pietsch, Larissa [VerfasserIn] |
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| | Saffrich, Rainer [VerfasserIn] |
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| | Wuchter, Patrick [VerfasserIn] |
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| | Walenda, Thomas [VerfasserIn] |
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| | Bork, Simone [VerfasserIn] |
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| | Horn, Patrick [VerfasserIn] |
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| | Diehlmann, Anke [VerfasserIn] |
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| | Eckstein, Volker [VerfasserIn] |
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| | Ho, Anthony Dick [VerfasserIn] |
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| | Wagner, Wolfgang [VerfasserIn] |
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| Titel: | N-Cadherin is expressed on human hematopoietic progenitor cells and mediates interaction with human mesenchymal stromal cells |
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| Verf.angabe: | Frederik Wein, Larissa Pietsch, Rainer Saffrich, Patrick Wuchter, Thomas Walenda, Simone Bork, Patrick Horn, Anke Diehlmann, Volker Eckstein, Anthony D. Ho, Wolfgang Wagner |
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| E-Jahr: | 2010 |
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| Jahr: | 4 January 2010 |
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| Umfang: | 13 S. |
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| Fussnoten: | Gesehen am 24.10.2023 |
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| Titel Quelle: | Enthalten in: Stem cell research |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier, 2007 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 4(2010), 2 vom: März, Seite 129-139 |
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| ISSN Quelle: | 1876-7753 |
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| Abstract: | Specific cell-cell junctions between hematopoietic stem cells (HSC) and their niche have been shown to regulate stem cell function. N-cadherin was suggested to play a central role in this process, whereas other studies indicated that it did not play an essential role in the murine model. We have analyzed the role of N-cadherin for interaction between hematopoietic progenitor cells (HPC) and supportive mesenchymal stromal cells (MSC) in a human-human setting. Expression of N-cadherin and of cadherin-11 (osteoblast cadherin) was analyzed in HPC by quantitative RT-PCR, Western blot, and flow cytometry. N-cadherin and cadherin-11 were expressed in HPC at a moderate level, whereas they were not detectable in differentiated cells. Confocal laser scanning microscopy revealed that N-cadherin and β-catenin are colocalized at the junction of HPC and MSC. siRNA knockdown of N-cadherin or cadherin-11 as well as treatment with the blocking function antibody decreased adhesive interaction of HPC to MSC. Furthermore, knockdown of N-cadherin or blocking function antibody impaired maintenance of long-term culture-initiating cells (LTC-IC) on coculture of HPC and MSC. These results indicate that N-cadherin is involved in the bidirectional interaction of human HPC with their cellular determinants in the niche. |
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| DOI: | doi:10.1016/j.scr.2009.12.004 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.scr.2009.12.004 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S1873506109001421 |
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| | DOI: https://doi.org/10.1016/j.scr.2009.12.004 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1867425564 |
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| Verknüpfungen: | → Zeitschrift |
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N-Cadherin is expressed on human hematopoietic progenitor cells and mediates interaction with human mesenchymal stromal cells / Wein, Frederik [VerfasserIn]; 4 January 2010 (Online-Ressource)
