| |  Online-Ressource |
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| Verfasst von: | Gabriel, Ute [VerfasserIn]  |
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| | Giehl, Michelle [VerfasserIn] |
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| | Prinzhorn, Wiltrud [VerfasserIn] |
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| | Trojan, Lutz [VerfasserIn] |
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| | Michel, Maurice Stephan [VerfasserIn] |
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| | Hofmann, Wolf-Karsten [VerfasserIn] |
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| | Seifarth, Wolfgang [VerfasserIn] |
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| | Fabarius, Alice [VerfasserIn] |
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| Titel: | Urine from current smokers induces centrosome aberrations and spindle defects in vitro in nonmalignant human cell lines |
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| Verf.angabe: | Ute Gabriel, Michelle Giehl, Wiltrud Haass, Lutz Trojan, Maurice Stephan Michel, Wolf-Karsten Hofmann, Wolfgang Seifarth, Alice Fabarius |
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| E-Jahr: | 2010 |
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| Jahr: | 13 December 2010 |
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| Umfang: | 10 S. |
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| Fussnoten: | Gesehen am 28.11.2023 |
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| Titel Quelle: | Enthalten in: Cancer genetics and cytogenetics |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1979 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 203(2010), 2 vom: Dez., Seite 253-262 |
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| ISSN Quelle: | 1873-4456 |
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| Abstract: | Tobacco smoke containing numerous derived chemical carcinogens is the main risk factor for urothelial carcinoma. These carcinogens can induce DNA damage leading to chromosomal instability, which plays a fundamental role in urothelial carcinogenesis. Possible mechanisms could be centrosomal aberrations, which cause defective spindles and may be responsible for genetic instability. We evaluated the effect of urine from never smokers (NS) and current smokers (CS) in concentrations of 0 to 50% on cell proliferation, chromosomes, centrosomes, and the spindle status of normal human dermal fibroblasts and normal human urothelial cells (UROtsa). After 2 weeks of urine treatment, cell cultures were analyzed by centrosome and spindle immunostaining and conventional cytogenetics. Effects were compared to results of untreated controls. Analysis of normal human dermal fibroblasts and UROtsa cells revealed that urine from CS induced higher values of centrosome aberrations in a dose-dependent and cell line-independent manner when compared to cultures treated with urine from NS and untreated controls. Centrosomal alterations correlated with spindle defects and an increase of sporadic chromosomal aberrations. The observations suggest a causative role of chemical carcinogens in urine from CS in the origin of centrosome and spindle defects in vitro leading to chromosomal instability and may be involved in urothelial carcinogenesis. |
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| DOI: | doi:10.1016/j.cancergencyto.2010.07.135 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.cancergencyto.2010.07.135 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S0165460810004486 |
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| | DOI: https://doi.org/10.1016/j.cancergencyto.2010.07.135 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Adult |
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| | Carcinogens |
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| | Cell Line |
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| | Cell Proliferation |
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| | Cells, Cultured |
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| | Centrosome |
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| | DNA Damage |
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| | Dose-Response Relationship, Drug |
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| | Fibroblasts |
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| | Humans |
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| | In Vitro Techniques |
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| | Middle Aged |
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| | Skin |
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| | Smoking |
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| | Spindle Apparatus |
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| | Urothelium |
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| K10plus-PPN: | 1871451132 |
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| Verknüpfungen: | → Zeitschrift |
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Urine from current smokers induces centrosome aberrations and spindle defects in vitro in nonmalignant human cell lines / Gabriel, Ute [VerfasserIn]; 13 December 2010 (Online-Ressource)
