| Online-Ressource |
Verfasst von: | Dickhut, Andrea [VerfasserIn]  |
| Dexheimer, Verena [VerfasserIn]  |
| Martin, Katja [VerfasserIn]  |
| Lauinger, Rebekka [VerfasserIn]  |
| Heisel, Jens Christian [VerfasserIn]  |
| Richter, Wiltrud [VerfasserIn]  |
Titel: | Chondrogenesis of human mesenchymal stem cells by local transforming growth factor-beta delivery in a biphasic resorbable carrier |
Verf.angabe: | Andrea Dickhut, Verena Dexheimer, Katja Martin, Rebekka Lauinger, Christian Heisel, Wiltrud Richter |
Jahr: | 2010 |
Umfang: | 12 S. |
Fussnoten: | Online veröffentlicht: 8 October, 2009 ; Gesehen am 14.02.2024 |
Titel Quelle: | Enthalten in: Tissue engineering / A |
Ort Quelle: | Larchmont, NY : Liebert, 2008 |
Jahr Quelle: | 2010 |
Band/Heft Quelle: | 16(2010), 2, Seite 453-464 |
ISSN Quelle: | 1937-335X |
Abstract: | Little is known about the potential of growth factor-augmented biphasic implants composed of a gel and a solid scaffold to enhance chondrogenesis of mesenchymal stem cells (MSCs). We analyzed whether a collagen type I/III carrier and fibrin glue (FG) combined to a biphasic construct support in vitro chondrogenesis of MSCs and allow for local release of bioactive transforming growth factor-beta1 (TGF-β1). Further, a possible advantage of partial autologous fibrin glue (PAF) over commercial FG was assessed. Collagen carriers seeded with 5 × 105 human MSCs with or without FG, PAF, or TGF-β1-upgraded FG were cultured for 6 weeks in chondrogenic medium with or without TGF-β1. Pellets with or without FG/PAF served as controls. FG and collagen carriers allowed strong upregulation of COL2A1, AGC, and COL10A1 mRNA, deposition of collagen-type II, and mediated a significantly higher proteoglycan content compared with biomaterial-free pellets. Collagen-carrier groups contained significantly more proteoglycan than FG and PAF pellets, whereas biphasic PAF-carrier constructs were inferior to FG-carrier constructs. Upgrading of biphasic FG-carrier constructs with 50 ng TGF-β1/construct mediated chondrogenesis as successfully as supply of TGF-β1 via the medium. In conclusion, the biphasic carrier constructs showed a high biofunctionality by continuous form stability with improved chondrogenesis and long-term local supply of bioactive TGF-β1 which may be useful to enhance matrix-assisted repair strategies for damaged cartilage. |
DOI: | doi:10.1089/ten.tea.2009.0168 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1089/ten.tea.2009.0168 |
| Volltext: https://www.liebertpub.com/doi/10.1089/ten.tea.2009.0168 |
| DOI: https://doi.org/10.1089/ten.tea.2009.0168 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1880745801 |
Verknüpfungen: | → Zeitschrift |
Chondrogenesis of human mesenchymal stem cells by local transforming growth factor-beta delivery in a biphasic resorbable carrier / Dickhut, Andrea [VerfasserIn]; 2010 (Online-Ressource)