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Verfasst von:Dickhut, Andrea [VerfasserIn]   i
 Dexheimer, Verena [VerfasserIn]   i
 Martin, Katja [VerfasserIn]   i
 Lauinger, Rebekka [VerfasserIn]   i
 Heisel, Jens Christian [VerfasserIn]   i
 Richter, Wiltrud [VerfasserIn]   i
Titel:Chondrogenesis of human mesenchymal stem cells by local transforming growth factor-beta delivery in a biphasic resorbable carrier
Verf.angabe:Andrea Dickhut, Verena Dexheimer, Katja Martin, Rebekka Lauinger, Christian Heisel, Wiltrud Richter
Jahr:2010
Umfang:12 S.
Fussnoten:Online veröffentlicht: 8 October, 2009 ; Gesehen am 14.02.2024
Titel Quelle:Enthalten in: Tissue engineering / A
Ort Quelle:Larchmont, NY : Liebert, 2008
Jahr Quelle:2010
Band/Heft Quelle:16(2010), 2, Seite 453-464
ISSN Quelle:1937-335X
Abstract:Little is known about the potential of growth factor-augmented biphasic implants composed of a gel and a solid scaffold to enhance chondrogenesis of mesenchymal stem cells (MSCs). We analyzed whether a collagen type I/III carrier and fibrin glue (FG) combined to a biphasic construct support in vitro chondrogenesis of MSCs and allow for local release of bioactive transforming growth factor-beta1 (TGF-β1). Further, a possible advantage of partial autologous fibrin glue (PAF) over commercial FG was assessed. Collagen carriers seeded with 5 × 105 human MSCs with or without FG, PAF, or TGF-β1-upgraded FG were cultured for 6 weeks in chondrogenic medium with or without TGF-β1. Pellets with or without FG/PAF served as controls. FG and collagen carriers allowed strong upregulation of COL2A1, AGC, and COL10A1 mRNA, deposition of collagen-type II, and mediated a significantly higher proteoglycan content compared with biomaterial-free pellets. Collagen-carrier groups contained significantly more proteoglycan than FG and PAF pellets, whereas biphasic PAF-carrier constructs were inferior to FG-carrier constructs. Upgrading of biphasic FG-carrier constructs with 50 ng TGF-β1/construct mediated chondrogenesis as successfully as supply of TGF-β1 via the medium. In conclusion, the biphasic carrier constructs showed a high biofunctionality by continuous form stability with improved chondrogenesis and long-term local supply of bioactive TGF-β1 which may be useful to enhance matrix-assisted repair strategies for damaged cartilage.
DOI:doi:10.1089/ten.tea.2009.0168
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1089/ten.tea.2009.0168
 Volltext: https://www.liebertpub.com/doi/10.1089/ten.tea.2009.0168
 DOI: https://doi.org/10.1089/ten.tea.2009.0168
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1880745801
Verknüpfungen:→ Zeitschrift

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