Identification of NAD-RNA species and ADPR-RNA decapping in Archaea

• Verfasst von: Gomes-Filho, José Vicente [VerfasserIn]
• Verfasst von: Breuer, Ruth [VerfasserIn]
• Verfasst von: Morales-Filloy, Hector Gabriel [VerfasserIn]
• Verfasst von: Pozhydaieva, Nadiia [VerfasserIn]
• Verfasst von: Borst, Andreas [VerfasserIn]
• Verfasst von: Paczia, Nicole [VerfasserIn]
• Verfasst von: Soppa, Jörg [VerfasserIn]
• Verfasst von: Höfer, Katharina [VerfasserIn]
• Verfasst von: Jäschke, Andres [VerfasserIn]
• Verfasst von: Randau, Lennart [VerfasserIn]
• Titel: Identification of NAD-RNA species and ADPR-RNA decapping in Archaea
• Verf.angabe: José Vicente Gomes-Filho, Ruth Breuer, Hector Gabriel Morales-Filloy, Nadiia Pozhydaieva, Andreas Borst, Nicole Paczia, Jörg Soppa, Katharina Höfer, Andres Jäschke & Lennart Randau
• E-Jahr: 2023
• Jahr: 21 November 2023
• Umfang: 12 S.
• Fussnoten: Gesehen am 13.03.2024
• Titel Quelle: Enthalten in: Nature Communications
• Ort Quelle: [London] : Springer Nature, 2010
• Jahr Quelle: 2023
• Band/Heft Quelle: 14(2023), Artikel-ID 7597, Seite 1-12
• ISSN Quelle: 2041-1723
• DOI: doi:10.1038/s41467-023-43377-x
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Archaeal biology
• Sach-SW: Archaeal physiology
• Sach-SW: RNA modification
• K10plus-PPN: 1883321220

Abstract

NAD is a coenzyme central to metabolism that also serves as a 5′-terminal cap for bacterial and eukaryotic transcripts. Thermal degradation of NAD can generate nicotinamide and ADP-ribose (ADPR). Here, we use LC-MS/MS and NAD captureSeq to detect and identify NAD-RNAs in the thermophilic model archaeon Sulfolobus acidocaldarius and in the halophilic mesophile Haloferax volcanii. None of the four Nudix proteins of S. acidocaldarius catalyze NAD-RNA decapping in vitro, but one of the proteins (Saci_NudT5) promotes ADPR-RNA decapping. NAD-RNAs are converted into ADPR-RNAs, which we detect in S. acidocaldarius total RNA. Deletion of the gene encoding the 5′−3′ exonuclease Saci-aCPSF2 leads to a 4.5-fold increase in NAD-RNA levels. We propose that the incorporation of NAD into RNA acts as a degradation marker for Saci-aCPSF2. In contrast, ADPR-RNA is processed by Saci_NudT5 into 5′-p-RNAs, providing another layer of regulation for RNA turnover in archaeal cells.