Ultra-sensitive FISH is a useful tool for studying chronic HIV-1 infection

• Verfasst von: Deichmann, Martin [VerfasserIn]
• Verfasst von: Bentz, Martin [VerfasserIn]
• Verfasst von: Haas, Rainer [VerfasserIn]
• Titel: Ultra-sensitive FISH is a useful tool for studying chronic HIV-1 infection
• Verf.angabe: Martin Deichmann, Martin Bentz, Rainer Haas
• Jahr: 1997
• Umfang: 7 S.
• Fussnoten: Elektronische Reproduktion der Druck-Ausgabe 23. November 1999 ; Gesehen am 03.04.2024
• Titel Quelle: Enthalten in: Journal of virological methods
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1980
• Jahr Quelle: 1997
• Band/Heft Quelle: 65(1997), 1, Seite 19-25
• ISSN Quelle: 1879-0984
• DOI: doi:10.1016/S0166-0934(96)02164-7
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: 8E5/LAV
• Sach-SW: Chronic infection
• Sach-SW: Human immunodeficiency virus-1 (HIV-1)
• Sach-SW: U1/HIV-1
• Sach-SW: Ultra-sensitive fluorescence in situ hybridization (FISH)
• K10plus-PPN: 1884923216

Abstract

Using an ultra-sensitive fluorescence in situ hybridization (FISH) protocol, integration sites of the human immunodeficiency virus-1 (HIV-1) were mapped in two model cell lines for chronic HIV infection. In the promonocytic cell line U1/HIV-1, the two integrated copies of proviral HIV-DNA were located on the short arm of one copy of chromosome X (band Xp21) and on a rearranged chromosome 6. In the T-cell line 8E5/LAV, the single copy of proviral HIV-DNA was integrated into chromosomal bands 13q14-q21, which are known to contain common fragile sites. In addition, ultra-sensitive FISH was used for the detection of HIV provirus sequences in interphase cells, achieving a high hybridization efficiency. This application allows the analysis of the HIV status on a single cell level and thus may become a useful tool for the study of HIV infection.