Identification of genetic imbalances in malignant lymphoma using comparative genomic hybridization

• Verfasst von: Bentz, Martin [VerfasserIn]
• Verfasst von: Döhner, Hartmut [VerfasserIn]
• Verfasst von: Werner, Claudius Alexander [VerfasserIn]
• Verfasst von: Huck, Karin [VerfasserIn]
• Verfasst von: Baudis, Michael [VerfasserIn]
• Verfasst von: Joos, Stefan [VerfasserIn]
• Verfasst von: Lichter, Peter [VerfasserIn]
• Verfasst von: Schlegelberger, Brigitte [VerfasserIn]
• Verfasst von: Trümper, Lorenz H. [VerfasserIn]
• Verfasst von: Pfreundschuh, Michael [VerfasserIn]
• Verfasst von: Feller, Alfred C. [VerfasserIn]
• Verfasst von: Möller, Peter [VerfasserIn]
• Titel: Identification of genetic imbalances in malignant lymphoma using comparative genomic hybridization
• Verf.angabe: Martin Bentz, Hartmut Döhner, Claudius A. Werner, Karin Huck, Michael Baudis, Stefan Joos, Peter Lichter, Brigitte Schlegelberger, Lorenz H. Trümper, Michael Pfreundschuh, Alfred C. Feller, Peter Möller
• E-Jahr: 1995
• Jahr: January 1995
• Umfang: 5 S.
• Fussnoten: Gesehen am 05.11.2024
• Titel Quelle: Enthalten in: Stem cells
• Ort Quelle: Oxford : Oxford University Press, 1993
• Jahr Quelle: 1995
• Band/Heft Quelle: 13(1995), suppl. 3, Seite 83-87
• ISSN Quelle: 1549-4918
• DOI: doi:10.1002/stem.5530130713
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1907627405

Abstract

In comparison to leukemias, the clinical relevance of chromosomal aberrations in non-Hodgkin's lymphoma (NHL) is not as well understood. This is primarily due to limitations of chromosomal banding techniques which have been the central methods for cytogenetic analysis. These techniques depend on the availability of fresh tumor tissue and the examination of metaphase cells which may not be representative for the major cell clone in vivo. In contrast, the new technique of comparative genomic hybridization (CGH) allows researchers to obtain a comprehensive view of chromosomal gains and losses by analyzing tumor DNA, which can be prepared from archival tissue samples. Results of CGH studies in three different types of lymphoproliferative disorders are outlined in this paper demonstrating that: (1) in chronic B cell leukemias, chromosomal aberrations are missed by banding analysis in a high proportion of cases, (2) CGH on paraffin-embedded tissue samples can be used for cytogenetic analysis within clinical multicenter trials and (3) DNA amplifications are more frequent in NHL than previously assumed. Thus, it can be expected that CGH will contribute both to the understanding of pathogenetic mechanisms and the identification of clinically relevant chromosome aberrations in NHL.