Fluorescence microscopy

• Titel: Fluorescence microscopy
• Titelzusatz: from principles to biological applications
• Mitwirkende: Kubitscheck, Ulrich [Hrsg.]
• Verf.angabe: ed. by Ulrich Kubitscheck
• Verlagsort: Weinheim [Germany]
• Verlag: Wiley-Blackwell
• Jahr: 2013
• Umfang: XX, 410 S.
• Illustrationen: Ill., graph. Darst.
• Fussnoten: Includes bibliographical references and index. - Electronic reproduction; Palo Alto, Calif; ebrary; 2013; Available via World Wide Web; Access may be limited to ebrary affiliated libraries
• ISBN: 978-3-527-32922-9
• ISBN: 978-3-527-67160-1
• ISBN: 978-3-527-67159-5
• Bestellnr.: 9781299464650
• Schlagwörter: (s)Fluoreszenzmikroskopie
• Schlagwörter: (s)Fluoreszenzmikroskopie
• Datenträger: Online-Ressource
• Sprache: eng
• (Gesamttitel): Myilibrary
• (Umfang): Online-Ressource
• (Ort): [S.l.]
• (Verlag): MyiLibrary
• Bibliogr. Hinweis: Erscheint auch als : Druck-Ausgabe: Fluorescence microscopy. - Weinheim : Wiley-Blackwell, 2013. - XX, 410 S.
• RVK-Notation: WC 2900
• K10plus-PPN: 1653016795
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

Intro -- Related Titles -- Title Page -- Copyright -- Preface -- List of Contributors -- Chapter 1: Introduction to Optics and Photophysics -- 1.1 Interference: Light as a Wave -- 1.2 Two Effects of Interference: Diffraction and Refraction -- 1.3 Optical Elements -- 1.4 The Far-Field, Near-Field, and Evanescent Waves -- 1.5 Optical Aberrations -- 1.6 Physical Background of Fluorescence -- 1.7 Photons, Poisson Statistics, and AntiBunching -- References -- Chapter 2: Principles of Light Microscopy -- 2.1 Introduction -- 2.2 Construction of Light Microscopes -- 2.3 Wave Optics and Resolution -- 2.4 Apertures, Pupils, and Telecentricity -- 2.5 Microscope Objectives -- 2.6 Contrast -- 2.7 Summary -- Acknowledgments -- References -- Chapter 3: Fluorescence Microscopy -- 3.1 Features of Fluorescence Microscopy -- 3.2 A Fluorescence Microscope -- 3.3 Types of Noise in a Digital Microscopy Image -- 3.4 Quantitative Fluorescence Microscopy -- 3.5 Limitations of Fluorescence Microscopy -- 3.6 Current Avenues of Development -- References -- Further Reading -- Recommended Internet Resources -- Fluorescent spectra database -- Chapter 4: Fluorescence Labeling -- 4.1 Introduction -- 4.2 Principles of Fluorescence -- 4.3 Key Properties of Fluorescent Labels -- 4.4 Synthetic Fluorophores -- 4.5 Genetically Encoded Labels -- 4.6 Label Selection for Particular Applications -- 4.7 Conclusions -- References -- Chapter 5: Confocal Microscopy -- 5.1 Introduction -- 5.2 The Theory of Confocal Microscopy -- 5.3 Applications of Confocal Microscopy -- Acknowledgments -- References -- Chapter 6: Fluorescence Photobleaching and Photoactivation Techniques -- 6.1 Introduction -- 6.2 Basic Concepts and Procedures -- 6.3 Fluorescence Recovery after Photobleaching (FRAP) -- 6.4 Continuous Fluorescence Microphotolysis (CFM) -- 6.5 Confocal Photobleaching.