The condensed protocols from molecular cloning: a laboratory manual

• Verfasst von: Sambrook, Joseph
• Verfasst von: Russell, David W.
• Titel: The condensed protocols from molecular cloning: a laboratory manual
• Verf.angabe: Joseph Sambrook; David W. Russell
• Verlagsort: Cold Spring Harbor, N.Y.
• Verlag: Cold Spring Harbor Laboratory Press
• E-Jahr: 2006
• Jahr: c2006
• Umfang: V, 800 S.
• Illustrationen: Ill., graph. Darst.
• Fussnoten: Includes index
• ISBN: 978-0-87969-772-3
• ISBN: 0-87969-772-5
• ISBN: 0-87969-771-7
• ISBN: 978-0-87969-771-6
• Schlagwörter: (s)Genklonierung
• Schlagwörter: (s)DNS-Klonierung
• Schlagwörter: (s)Genklonierung
• Sprache: eng
• Sonstige Nr.: Identnummer: 2005022077
• RVK-Notation: WC 4460
• K10plus-PPN: 504464205

Abstract

Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 -- Working with high-capacity vectors -- Gas electrophoresisof DNA and pulsed-field agarose -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.