Effects of ethanol on the properties of platelets and endothelial cells in model experiments

• Verfasst von: Stach-Jablonski, Ksenija [VerfasserIn]
• Verfasst von: Kälsch, Anna-Isabelle [VerfasserIn]
• Verfasst von: Weiß, Christel [VerfasserIn]
• Verfasst von: Elmas, Elif [VerfasserIn]
• Verfasst von: Borggrefe, Martin [VerfasserIn]
• Verfasst von: Kälsch, Thorsten [VerfasserIn]
• Titel: Effects of ethanol on the properties of platelets and endothelial cells in model experiments
• Verf.angabe: Ksenija Stach, Anna-Isabelle Kälsch, Christel Weiß, Elif Elmas, Martin Borggrefe, Thorsten Kälsch
• E-Jahr: 2012
• Jahr: June 26, 2012
• Umfang: 5 S.
• Fussnoten: Published online: June 26, 2012 ; Gesehen am 09.08.2018
• Titel Quelle: Enthalten in: World journal of cardiology
• Ort Quelle: Hong Kong : Baishideng Publ., 2009
• Jahr Quelle: 2012
• Band/Heft Quelle: 4(2012), 6, Seite 201-205
• ISSN Quelle: 1949-8462
• DOI: doi:10.4330/wjc.v4.i6.201
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1578424496

Abstract

Aim: To investigate effects of ethanol on activity markers of atherosclerosis in an in vitro endothelial cell model. Methods: After 24 h incubation with ethanol (0.0095%), human umbilical vein endothelial cells were stimulated for 1 h with lipopolysaccharide, and were then incubated in direct contact with activated platelets. Following this incubation, the expression of CD40L and CD62P on platelets, and the expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), urokinase plasminogen activator receptor (uPAR), and membrane-type 1 matrix metalloproteinase (MT1-MMP) on endothelial cells were measured by flow cytometry. Results: The increased expression of VCAM-1 and uPAR on endothelial cells by proinflammatory stimulation with activated platelets was significantly reduced through pre-incubation with ethanol (P < 0.05). Furthermore, platelets in direct contact with ethanol and with endothelial cells pre-incubated in ethanol showed a significant reduction in their CD40L expression (P < 0.05). Ethanol had no significant effect on ICAM-1 and MT1-MMP expression on endothelial cells. Conclusion: Ethanol directly attenuates platelet activation and has significant endothelial cell-mediated effects on selected markers of atherosclerosis in vitro. These findings underline possible protective effects of ethanol on atherosclerosis.