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Verfasst von:Suda, Arnold [VerfasserIn]   i
 Tinelli, Marco [VerfasserIn]   i
 Beisemann, Nils [VerfasserIn]   i
 Bischel, Oliver E. [VerfasserIn]   i
Titel:Diagnosis of periprosthetic joint infection using alpha-defensin test or multiplex-PCR
Titelzusatz:ideal diagnostic test still not found
Verf.angabe:Arnold J. Suda, Marco Tinelli, Nils D. Beisemann, Yoram Weil, Amal Khoury, Oliver E. Bischel
E-Jahr:2017
Jahr:4 February 2017
Umfang:7 S.
Fussnoten:Gesehen am 27.08.2018
Titel Quelle:Enthalten in: International orthopaedics
Ort Quelle:Berlin : Springer, 1977
Jahr Quelle:2017
Band/Heft Quelle:41(2017), 7, Seite 1307-1313
ISSN Quelle:1432-5195
Abstract:Purpose: Diagnosing periprosthetic infection remains a challenge. Multiplex-PCR and biomarkers such as alpha-defensin are potentially useful and fast methods for detecting periprosthetic infection. This study compared these new methods with clinical assessment, conventional microbiological methods and histo-pathological examination. Methods: Twenty-eight consecutive patients with 30 joints and a mean age of 67.7 years (range 39 to 88) with removal of total hip arthroplasty (THA) or total knee replacement (TKR) were included in this study. Patients were classified according to the modified Musculoskeletal Infection Society score (MSIS) for infected joints. Punction fluid and tissue specimens were taken for conventional microbiological examination, alphadefensin test was performed, a synovial membrane specimen was used for multiplex-PCR and histopathological examination was carried out. Results: The alpha-defensin test and multiplex-PCR showed a sensitivity of 76.9 vs. 30.8% and a specificity of 82.4 vs. 100%, respectively. We found a significant difference between the positive and negative results (p = 0.0023). The conventional microbiological methods were not significantly different from the alpha-defensin test (p = 0.244) with a sensitivity of 84.6% and a specificity of 100% but did differ significantly from the multiplex PCR (p = 0.0030). There was a significant difference between modified MSIS classification and multiplex PCR (p = 0.0007). Conclusions: Neither alpha-defensin test nor multiplex-PCR could detect periprosthetic infection immediately and reliably. Multiplex-PCR was suitable for detecting the non-infected but not the truly infected. Alpha-defensin test was helpful but showed no satisfactory results. Conventional microbiological methods remain the most reliable for periprosthetic infection diagnosis.
DOI:doi:10.1007/s00264-017-3412-7
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: http://dx.doi.org/10.1007/s00264-017-3412-7
 Volltext: https://doi.org/10.1007/s00264-017-3412-7
 DOI: https://doi.org/10.1007/s00264-017-3412-7
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Alfa defensin
 Multiplex PCR
 Periprosthetic joint infection
K10plus-PPN:1580399576
Verknüpfungen:→ Zeitschrift

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