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| Verfasst von: | Bejarano Acuña, David Alejandro [VerfasserIn]  |
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| | Peng, Ke [VerfasserIn] |
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| | Laketa, Vibor [VerfasserIn] |
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| | Börner, Kathleen [VerfasserIn] |
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| | Jost, Katharina Laurence [VerfasserIn] |
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| | Lucic, Bojana [VerfasserIn] |
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| | Lusic, Marina [VerfasserIn] |
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| | Müller, Barbara [VerfasserIn] |
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| | Kräusslich, Hans-Georg [VerfasserIn] |
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| Titel: | HIV-1 nuclear import in macrophages is regulated by CPSF6-capsid interactions at the nuclear pore complex |
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| Verf.angabe: | David Alejandro Bejarano, Ke Peng, Vibor Laketa, Kathleen Börner, K. Laurence Jost, Bojana Lucic, Bärbel Glass, Marina Lusic, Barbara Müller, Hans-Georg Kräusslich |
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| E-Jahr: | 2019 |
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| Jahr: | 23 January 2019 |
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| Umfang: | 31 S. |
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| Fussnoten: | Gesehen am 11.07.2019 |
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| Titel Quelle: | Enthalten in: eLife |
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| Ort Quelle: | Cambridge : eLife Sciences Publications, 2012 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 8(2019) Artikel-Nummer e41800, 31 Seiten |
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| ISSN Quelle: | 2050-084X |
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| Abstract: | Nuclear entry of HIV-1 replication complexes through intact nuclear pore complexes is critical for successful infection. The host protein cleavage-and-polyadenylation-specificity-factor-6 (CPSF6) has been implicated in different stages of early HIV-1 replication. Applying quantitative microscopy of HIV-1 reverse-transcription and pre-integration-complexes (RTC/PIC), we show that CPSF6 is strongly recruited to nuclear replication complexes but absent from cytoplasmic RTC/PIC in primary human macrophages. Depletion of CPSF6 or lack of CPSF6 binding led to accumulation of HIV-1 subviral complexes at the nuclear envelope of macrophages and reduced infectivity. Two-color stimulated-emission-depletion microscopy indicated that under these circumstances HIV-1 complexes are retained inside the nuclear pore and undergo CA-multimer dependent CPSF6 clustering adjacent to the nuclear basket. We propose that nuclear entry of HIV-1 subviral complexes in macrophages is mediated by consecutive binding of Nup153 and CPSF6 to the hexameric CA lattice. |
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| DOI: | doi:10.7554/eLife.41800 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.7554/eLife.41800 |
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| | DOI: https://doi.org/10.7554/eLife.41800 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | CPSF6 |
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| | Human immunodeficiency virus |
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| | macrophages |
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| | nuclear import |
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| | nuclear pore complex |
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| | pre-integration complex |
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| K10plus-PPN: | 1669021815 |
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| Verknüpfungen: | → Zeitschrift |
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HIV-1 nuclear import in macrophages is regulated by CPSF6-capsid interactions at the nuclear pore complex / Bejarano Acuña, David Alejandro [VerfasserIn]; 23 January 2019 (Online-Ressource)
