Perilipin 1 expression differentiates liposarcoma from other types of soft tissue sarcoma

• Verfasst von: Straub, Beate Katharina [VerfasserIn]
• Verfasst von: Pawella, Lena Maria [VerfasserIn]
• Verfasst von: Renner, Marcus [VerfasserIn]
• Verfasst von: Eiteneuer, Eva [VerfasserIn]
• Verfasst von: Hashani, Merita [VerfasserIn]
• Verfasst von: Schirmacher, Peter [VerfasserIn]
• Verfasst von: Mechtersheimer, Gunhild [VerfasserIn]
• Titel: Perilipin 1 expression differentiates liposarcoma from other types of soft tissue sarcoma
• Verf.angabe: Beate K. Straub, Hagen R. Witzel, Lena M. Pawella, Marcus Renner, Eva Eiteneuer, Merita Hashani, Peter Schirmacher, Wilfried Roth, and Gunhild Mechtersheimer
• E-Jahr: 2019
• Jahr: 22 May 2019
• Umfang: 12 S.
• Fussnoten: Gesehen am 12.09.2019
• Titel Quelle: Enthalten in: The American journal of pathology
• Ort Quelle: New York [u.a.] : Elsevier, 1925
• Jahr Quelle: 2019
• Band/Heft Quelle: 189(2019), 8, Seite 1547-1558
• ISSN Quelle: 1525-2191
• DOI: doi:10.1016/j.ajpath.2019.04.017
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1676770798

Abstract

Lipid droplets, a morphologic feature of adipocytic tumors, are strongly regulated by associated proteins of the perilipin/PAT (perilipin, adipophilin, and tail-interacting protein of 47 kD) family. So far, the use of perilipins as markers for differential diagnosis of soft tissue tumors has only been studied in a few cases. The aim of this study was to investigate the expression of perilipins in 478 human soft tissue tumors and 60 respective normal tissues. Perilipin 1 was immunohistochemically positive in all studied cases of well-differentiated liposarcomas, >90% of myxoid round cell liposarcomas, and >70% of pleomorphic liposarcomas, whereas only the differentiated components of dedifferentiated liposarcomas were immunohistochemically positive for perilipin 1. All other types of soft tissue sarcomas were negative for perilipin 1. Perilipin 2 was more prominent in dedifferentiated and pleomorphic liposarcomas and nearly all other high-grade sarcomas. In well-differentiated liposarcomas, lipomas, or normal adipose tissue, perilipin 2 was virtually absent. In addition, long-term stimulation of adipogenesis in the liposarcoma cell line LiSa-2 restored perilipin 1 expression, as exhibited in the source tumor. Furthermore, knockdown of perilipin 2 or perilipin 3 in LiSa-2 cells influenced lipid droplet number and size as well as cell vitality. In summary, perilipin 1 is a promising marker for the differential diagnosis of liposarcomas from other soft tissue sarcomas, whereas perilipin 2 correlates negatively with tumor grade and may be therapeutically useful.