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| Verfasst von: | Dimitrova-Paternoga, Lyudmila Nikolova [VerfasserIn]  |
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| | Valkov, Eugene [VerfasserIn] |
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| | Aibara, Shintaro [VerfasserIn] |
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| | Flemming, Dirk [VerfasserIn] |
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| | McLaughlin, Stephen H. [VerfasserIn] |
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| | Hurt, Ed [VerfasserIn] |
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| | Stewart, Murray [VerfasserIn] |
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| Titel: | Structural characterization of the Chaetomium thermophilum TREX-2 complex and its interaction with the mRNA nuclear export factor Mex67:Mtr2 |
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| Verf.angabe: | Lyudmila Dimitrova, Eugene Valkov, Shintaro Aibara, Dirk Flemming, Stephen H. McLaughlin, Ed Hurt, and Murray Stewart |
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| E-Jahr: | 2015 |
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| Jahr: | June 4, 2015 |
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| Umfang: | 12 S. |
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| Fussnoten: | Gesehen am 28.05.2020 |
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| Titel Quelle: | Enthalten in: Structure |
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| Ort Quelle: | London [u.a.] : Elsevier Science, 1993 |
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| Jahr Quelle: | 2015 |
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| Band/Heft Quelle: | 23(2015), 7, Seite 1246-1257 |
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| ISSN Quelle: | 1878-4186 |
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| Abstract: | The TREX-2 complex integrates mRNA nuclear export into the gene expression pathway and is based on a Sac3 scaffold to which Thp1, Sem1, Sus1, and Cdc31 bind. TREX-2 also binds the mRNA nuclear export factor, Mex67:Mtr2, through the Sac3 N-terminal region (Sac3N). Here, we characterize Chaetomium thermophilum TREX-2, show that the in vitro reconstituted complex has an annular structure, and define the structural basis for interactions between Sac3, Sus1, Cdc31, and Mex67:Mtr2. Crystal structures show that the binding of C. thermophilum Sac3N to the Mex67 NTF2-like domain (Mex67NTF2L) is mediated primarily through phenylalanine residues present in a series of repeating sequence motifs that resemble those seen in many nucleoporins, and Mlp1 also binds Mex67:Mtr2 using a similar motif. Deletion of Sac3N generated growth and mRNA export defects in Saccharomyces cerevisiae, and we propose TREX-2 and Mlp1 function to facilitate export by concentrating mature messenger ribonucleoparticles at the nuclear pore entrance. |
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| DOI: | doi:10.1016/j.str.2015.05.002 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.str.2015.05.002 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S096921261500180X |
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| | DOI: https://doi.org/10.1016/j.str.2015.05.002 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1698993919 |
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| Verknüpfungen: | → Zeitschrift |
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Structural characterization of the Chaetomium thermophilum TREX-2 complex and its interaction with the mRNA nuclear export factor Mex67:Mtr2 / Dimitrova-Paternoga, Lyudmila Nikolova [VerfasserIn]; June 4, 2015 (Online-Ressource)
