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| Verfasst von: | Jing, Cui [VerfasserIn]  |
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| | Oehrl, Stephanie [VerfasserIn] |
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| | Ahmad, Fareed [VerfasserIn] |
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| | Brenner, Thorsten [VerfasserIn] |
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| | Uhle, Florian [VerfasserIn] |
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| | Nußhag, Christian [VerfasserIn] |
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| | Funck, Felix [VerfasserIn] |
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| | Weigand, Markus A. [VerfasserIn] |
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| | Morath, Christian [VerfasserIn] |
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| | Schäkel, Knut [VerfasserIn] |
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| Titel: | Detection of in vivo inflammasome activation for predicting sepsis mortality |
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| Verf.angabe: | Jing Cui, Stephanie Oehrl, Fareed Ahmad, Thorsten Brenner, Florian Uhle, Christian Nusshag, Christoph Rupp, Felix Funck, Stefan Meisel, Markus A. Weigand, Christian Morath and Knut Schäkel |
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| E-Jahr: | 2021 |
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| Jahr: | 04 February 2021 |
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| Umfang: | 11 S. |
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| Fussnoten: | Gesehen am 01.12.2021 |
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| Titel Quelle: | Enthalten in: Frontiers in immunology |
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| Ort Quelle: | Lausanne : Frontiers Media, 2010 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 11(2021), Artikel-ID 613745, Seite 1-11 |
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| ISSN Quelle: | 1664-3224 |
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| Abstract: | Sepsis is a severe life-threatening syndrome caused by dysregulated host responses to infection. Biomarkers that allow for monitoring the patient’s immune status are needed. Recently, a flow cytometry-based detection of in vivo inflammasome activation by formation of cytoplasmic aggregates of ASC (apoptosis-associated speck-like protein containing a caspase recruitment domain) has been proposed. Here we report on the frequency of ASC-speck+ leukocytes correlating with the survival of sepsis. 25 patients with sepsis were sampled consecutively for 7 days. Blood, serum samples and patient data were collected according to the guidelines of the PredARRT-Sep-Trial. Flow cytometric analysis was performed on fresh whole blood samples to investigate the formation of ASC-specks in leukocyte subsets. Serum samples were analyzed for production of IL-1ß, IL-18 and additional inflammatory markers. ASC-speck formation was found to be increased in leukocytes from sepsis patients compared to healthy donor controls. The absolute number of ASC-speck+ neutrophils peaked on day 1. For monocytes, the highest percentage and maximum absolute number of ASC-speck+ cells were detected on day 6 and day 7. Inflammatory cytokines were elevated on day 1 and declined thereafter, with exception of IL-18. Survival analysis showed that patients with lower absolute numbers of ASC-speck+ monocytes (<1650 cells/ml) on day 6 had a lower probability to survive, with an hazard ratio (HR) of 10.178. Thus, the frequency of ASC-speck+ monocytes on day 6 after onset of sepsis may serve to identify patients at risk of death from sepsis. |
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| DOI: | doi:10.3389/fimmu.2020.613745 |
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| URL: | kostenfrei: Volltext ; Verlag: https://doi.org/10.3389/fimmu.2020.613745 |
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| | kostenfrei: Volltext: https://www.frontiersin.org/articles/10.3389/fimmu.2020.613745/full |
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| | DOI: https://doi.org/10.3389/fimmu.2020.613745 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | ASC-speck |
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| | biomarker Running Title |
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| | Inflammasome |
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| | Monocytes |
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| | Sepsis |
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| K10plus-PPN: | 1751089568 |
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| Verknüpfungen: | → Zeitschrift |
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| Lokale URL UB: |  Zum Volltext |
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Detection of in vivo inflammasome activation for predicting sepsis mortality / Jing, Cui [VerfasserIn]; 04 February 2021 (Online-Ressource)
