FADD/MORT1 and caspase-8 are recruited to TRAIL receptors 1 and 2 and are essential for apoptosis mediated by TRAIL receptor 2

• Verfasst von: Sprick, Martin [VerfasserIn]
• Verfasst von: Weigand, Markus A. [VerfasserIn]
• Verfasst von: Krammer, Peter H. [VerfasserIn]
• Verfasst von: Walczak, Henning [VerfasserIn]
• Titel: FADD/MORT1 and caspase-8 are recruited to TRAIL receptors 1 and 2 and are essential for apoptosis mediated by TRAIL receptor 2
• Verf.angabe: Martin R. Sprick, Markus A. Weigand, Eva Rieser, Charles T. Rauch, Peter Juo, John Blenis, Peter H. Krammer, and Henning Walczak
• Jahr: 2000
• Umfang: 11 S.
• Fussnoten: Elektronische Reproduktion der Druckausgabe ; Gesehen am 26.04.2021
• Titel Quelle: Enthalten in: Immunity
• Ort Quelle: [Cambridge, Mass.] : Cell Press, 1994
• Jahr Quelle: 2000
• Band/Heft Quelle: 12(2000), 6, Seite 599-609
• ISSN Quelle: 1097-4180
• DOI: doi:10.1016/S1074-7613(00)80211-3
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1755941463

Abstract

Apoptosis induced by tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL/APO-2L) has been shown to exert important functions during various immunological processes. The involvement of the death adaptor proteins FADD/MORT1, TRADD, and RIP and the apoptosis-initiating caspases-8 and -10 in death signaling by the two death-inducing TRAIL receptors 1 and 2 (TRAIL-R1 and TRAIL-R2) are controversial. Analysis of the native TRAIL death-inducing signaling complex (DISC) revealed ligand-dependent recruitment of FADD/MORT1 and caspase-8. Differential precipitation of ligand-stimulated TRAIL receptors demonstrated that FADD/MORT1 and caspase-8 were recruited to TRAIL-R1 and TRAIL-R2 independently of each other. FADD/MORT1- and caspase-8-deficient Jurkat cells expressing only TRAIL-R2 were resistant to TRAIL-induced apoptosis. Thus, FADD/MORT1 and caspase-8 are essential for apoptosis induction via TRAIL-R2.