Functionalization of cellular membranes with DNA nanotechnology

• Verfasst von: Schönit, Andreas [VerfasserIn]
• Verfasst von: Cavalcanti-Adam, Elisabetta A. [VerfasserIn]
• Verfasst von: Göpfrich, Kerstin [VerfasserIn]
• Titel: Functionalization of cellular membranes with DNA nanotechnology
• Verf.angabe: Andreas Schoenit, Elisabetta Ada Cavalcanti-Adam, and Kerstin Göpfrich
• E-Jahr: 2021
• Jahr: 12 March 2021
• Umfang: 13 S.
• Fussnoten: Gesehen am 19.11.2021
• Titel Quelle: Enthalten in: Trends in biotechnology
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1983
• Jahr Quelle: 2021
• Band/Heft Quelle: 39(2021), 11, Seite 1208-1220
• ISSN Quelle: 1879-3096
• DOI: doi:10.1016/j.tibtech.2021.02.002
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: cell biology
• Sach-SW: cellular plasma membrane
• Sach-SW: DNA nanotechnology
• Sach-SW: DNA origami
• Sach-SW: lipid bilayer
• Sach-SW: membrane engineering
• K10plus-PPN: 1778001297

Abstract

Due to its versatility and programmability, DNA nanotechnology has greatly expanded the experimental toolbox for biomedical research. Recent advances allow reliable and efficient functionalization of cellular plasma membranes with a variety of synthetic DNA constructs, ranging from single strands to complex 3D DNA origami. The scope for applications, which probe biophysical parameters or equip cells with novel functions, is rapidly increasing. These applications extend from programmed cellular connectivity and tissue engineering to molecular force measurements, controlled receptor-ligand interactions, membrane-anchored biosensors, and artificial transmembrane structures. Here, we give guidance on different strategies to functionalize cellular membranes with DNA nanotechnology and summarize current trends employing membrane-anchored DNA as a tool in biophysics, cell biology, and synthetic biology.