Development of high-throughput multiplex serology to detect serum antibodies against Coxiella burnetii

• Verfasst von: Jeske, Rima [VerfasserIn]
• Verfasst von: Dangel, Larissa [VerfasserIn]
• Verfasst von: Sauerbrey, Leander [VerfasserIn]
• Verfasst von: Frangoulidis, Dimitrios [VerfasserIn]
• Verfasst von: Teras, Lauren R. [VerfasserIn]
• Verfasst von: Fischer, Silke F. [VerfasserIn]
• Verfasst von: Waterboer, Tim [VerfasserIn]
• Titel: Development of high-throughput multiplex serology to detect serum antibodies against Coxiella burnetii
• Verf.angabe: Rima Jeske, Larissa Dangel, Leander Sauerbrey, Dimitrios Frangoulidis, Lauren R. Teras, Silke F. Fischer and Tim Waterboer
• E-Jahr: 2021
• Jahr: 17 November 2021
• Umfang: 11 S.
• Fussnoten: Gesehen am 19.01.2022
• Titel Quelle: Enthalten in: Microorganisms
• Ort Quelle: Basel : MDPI, 2013
• Jahr Quelle: 2021
• Band/Heft Quelle: 9(2021), 11, Artikel-ID 2373, Seite 1-11
• ISSN Quelle: 2076-2607
• DOI: doi:10.3390/microorganisms9112373
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Coxiella burnetii
• Sach-SW: infection marker
• Sach-SW: multiplex serology
• Sach-SW: seroepidemiology
• K10plus-PPN: 1786454947

Abstract

The causative agent of Q fever, the bacterium Coxiella burnetii (C. burnetii), has gained increasing interest due to outbreak events and reports about it being a potential risk factor for the development of lymphomas. In order to conduct large-scale studies for population monitoring and to investigate possible associations more closely, accurate and cost-effective high-throughput assays are highly desired. To address this need, nine C. burnetii proteins were expressed as recombinant antigens for multiplex serology. This technique enables the quantitative high-throughput detection of antibodies to multiple antigens simultaneously in a single reaction. Based on a reference group of 76 seropositive and 91 seronegative sera, three antigens were able to detect C. burnetii infections. Com1, GroEL, and DnaK achieved specificities of 93%, 69%, and 77% and sensitivities of 64%, 72%, and 47%, respectively. Double positivity to Com1 and GroEL led to a combined specificity of 90% and a sensitivity of 71%. In a subgroup of seropositives with an increased risk for chronic Q fever, the double positivity to these markers reached a specificity of 90% and a sensitivity of 86%. Multiplex serology enables the detection of antibodies against C. burnetii and appears well-suited to investigate associations between C. burnetii infections and the clinical manifestations in large-scale studies.