Absence of anti-glomerular basement membrane antibodies in 200 patients with systemic lupus erythematosus with or without lupus nephritis

• Verfasst von: Bourse Chalvon, Nellie [VerfasserIn]
• Verfasst von: Orquevaux, Pauline [VerfasserIn]
• Verfasst von: Giusti, Delphine [VerfasserIn]
• Verfasst von: Gatouillat, Gregory [VerfasserIn]
• Verfasst von: Tabary, Thierry [VerfasserIn]
• Verfasst von: Tonye Libyh, Marcelle [VerfasserIn]
• Verfasst von: Chrusciel, Jan [VerfasserIn]
• Verfasst von: Drame, Moustapha [VerfasserIn]
• Verfasst von: Stockton-Bliard, Grace [VerfasserIn]
• Verfasst von: Amoura, Zahir [VerfasserIn]
• Verfasst von: Arnaud, Laurent [VerfasserIn]
• Verfasst von: Lorenz, Hanns-Martin [VerfasserIn]
• Verfasst von: Blaison, Gilles [VerfasserIn]
• Verfasst von: Bonnotte, Bernard [VerfasserIn]
• Verfasst von: Magy-Bertrand, Nadine [VerfasserIn]
• Verfasst von: Revuz, Sabine [VerfasserIn]
• Verfasst von: Voll, Reinhard Edmund [VerfasserIn]
• Verfasst von: Hinschberger, Oliver [VerfasserIn]
• Verfasst von: Schwarting, Andreas [VerfasserIn]
• Verfasst von: Pham, Bach Nga [VerfasserIn]
• Verfasst von: Martin, Thierry [VerfasserIn]
• Verfasst von: Pennaforte, Jean-Loup [VerfasserIn]
• Verfasst von: Servettaz, Amelie [VerfasserIn]
• Titel: Absence of anti-glomerular basement membrane antibodies in 200 patients with systemic lupus erythematosus with or without lupus nephritis
• Titelzusatz: results of the GOODLUPUS study
• Verf.angabe: Nellie Bourse Chalvon, Pauline Orquevaux, Delphine Giusti, Gregory Gatouillat, Thierry Tabary, Marcelle Tonye Libyh, Jan Chrusciel, Moustapha Drame, Grace Stockton-Bliard, Zahir Amoura, Laurent Arnaud, Hanns-Martin Lorenz, Gilles Blaison, Bernard Bonnotte, Nadine Magy-Bertrand, Sabine Revuz, Reinhard Edmund Voll, Oliver Hinschberger, Andreas Schwarting, Bach Nga Pham, Thierry Martin, Jean-Loup Pennaforte and Amelie Servettaz
• E-Jahr: 2020
• Jahr: 14 December 2020
• Umfang: 9 S.
• Fussnoten: Gesehen am 03.03.2022
• Titel Quelle: Enthalten in: Frontiers in immunology
• Ort Quelle: Lausanne : Frontiers Media, 2010
• Jahr Quelle: 2020
• Band/Heft Quelle: 11(2020), Artikel-ID 597863, Seite 1-9
• ISSN Quelle: 1664-3224
• DOI: doi:10.3389/fimmu.2020.597863
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1788504585

Abstract

Introduction Anti-glomerular basement membrane (GBM) antibodies are pathogenic antibodies first detected in renal-limited anti-GBM disease and in Goodpasture disease, the latter characterized by rapidly progressive crescentic glomerulonephritis combined with intra-alveolar hemorrhage. Studies have suggested that anti-GBM antibody positivity may be of interest in lupus nephritis (LN). Moreover, severe anti-GBM vasculitis cases in patients with systemic lupus erythematosus (SLE) have been described in the literature, but few studies have assessed the incidence of anti-GBM antibodies in SLE patients.ObjectiveThe main study objective was to determine if positive anti-GBM antibodies were present in the serum of SLE patients with or without proliferative renal damage and compared to a healthy control group.MethodologyThis retrospective study was performed on SLE patients’ sera from a Franco-German European biobank, developed between 2011 and 2014, from 17 hospital centers in the Haut-Rhin region. Patients were selected according to their renal involvement, and matched by age and gender. The serum from healthy voluntary blood donors was also tested. Anti-GBM were screened by fluorescence enzyme immunoassay (FEIA), and then by indirect immunofluorescence (IIF) in case of low reactivity detection (titer >6 U/ml).ResultsThe cohort was composed of 100 SLE patients with proliferative LN (27% with class III, 67% with class IV, and 6% with class V), compared to 100 SLE patients without LN and 100 controls. Patients were mostly Caucasian and met the ACR 1997 criteria and/or the SLICC 2012 criteria. Among the 300 tested sera, no significant levels of anti-GBM antibodies were detected (>10 U/ml) by the automated technique, three sera were found “ambivalent” (>7 U/ml): one in the SLE with LN group and two in the SLE without LN group. Subsequent IIF assays did not detect anti-GBM antibodies.ConclusionAnti-GBM antibodies were not detected in the serum of Caucasian patients with SLE, even in case of renal involvement, a situation favoring the antigenic exposure of glomerular basement membranes. Our results reaffirm the central role of anti-GBM antibodies as a specific diagnostic biomarker for Goodpasture vasculitis and therefore confirm that anti-GBM antibody must not be carried out in patients with SLE (with or without LN) in the absence of disease-suggestive symptoms.