Microinjection of alpha-MSH followed by UV-irradiation blocks HSP 72 in human keratinocytes

• Verfasst von: Held, Björn Olaf [VerfasserIn]
• Verfasst von: Amato, Susanne [VerfasserIn]
• Verfasst von: Jung, Ernst G. [VerfasserIn]
• Verfasst von: Bayerl, Christiane [VerfasserIn]
• Titel: Microinjection of alpha-MSH followed by UV-irradiation blocks HSP 72 in human keratinocytes
• Verf.angabe: Björn Held, Susanne Amato, Ernst G. Jung, and Christiane Bayerl
• Jahr: 1999
• Umfang: 5 S.
• Fussnoten: Elektronische Reproduktion der Druck-Ausgabe ; First published: 06 February 2006 ; Gesehen am 23.05.2022
• Titel Quelle: Enthalten in: New York Academy of SciencesAnnals of the New York Academy of Sciences
• Ort Quelle: Oxford [u.a.] : Wiley-Blackwell, 1877
• Jahr Quelle: 1999
• Band/Heft Quelle: 885(1999), Seite 400-404
• ISSN Quelle: 1749-6632
• DOI: doi:10.1111/j.1749-6632.1999.tb08699.x
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1804097934

Abstract

The aim of this study was to investigate the interaction of alpha-melanocyte-stimulating hormone (α-MSH) and heat shock protein 72 (HSP 72) in human keratinocytes in order to learn about the link between the neuroimmunocutaneus system and UV-induced immunomodulation. 1-3α-MSH, a proopiomelanocortin (POMC) derived neuropeptide, 4 is produced in human keratinocytes 5, 6 as well as in normal and pathologic human skin. 7 It is upregulated by UV-irradiation, 8 and it is a potent mediator of inflammation 9, 10 and immune response. 3, 11 HSP 72, a chaperoning protein that has protective properties on cells, 12, 13 is upregulated by environmental stresses such as heat, UV-irradiation, drugs, and chemicals. 12, 14 Orel et al., 13 showed that there are inverse interactions between α-MSH and HSP 72 in the HaCat cell line. Therefore, we microinjected α-MSH into primary human keratinocytes in culture so as to study changes in HSP 72 at the protein level.